MCQ On HPLC With Answers

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Table of Contents

#1. What does HPLC stand for in the context of a QC lab in a pharmaceutical plant?

A) High Performance Liquid Chromatography

B) High Pressure Liquid Chromatography

C) High Precision Liquid Chromatography

D) High Purity Liquid Chromatography

#2. What is the primary purpose of HPLC in pharmaceutical quality control?

A) Weighing samples

B) Identifying impurities

C) Measuring pH levels

D) Conducting microbial analysis

#3. What is the role of the mobile phase in an HPLC analysis?

A) Separating compounds

B) Measuring pressure

C) Injecting samples

D) Recording data

#4. Which HPLC parameter is responsible for controlling the flow rate of the mobile phase?

A) Detector sensitivity

B) Pump pressure

C) Column temperature

D) Injection volume

#5. What does the term "retention time" refer to in the context of HPLC analysis?

A) Time taken for sample injection

B) Time taken for a compound to travel through the column

C) Time taken for column equilibration

D) Time taken for detector response

#6. . What is the purpose of the autosampler in an HPLC system?

A) Separating compounds

B) Injecting samples automatically

C) Controlling pump pressure

D) Monitoring column temperature

#7. Which type of HPLC column packing material is most commonly used for pharmaceutical applications?

A) Polymer-based

B) Glass-based

C) Silica-based

D) Metal-based

#8. In HPLC terminology, what does "gradient elution" refer to?

A) Changing the column temperature

B) Varying the mobile phase composition during analysis

C) Adjusting the detector sensitivity

D) Controlling the autosampler speed

#9. Which parameter is crucial for ensuring the reproducibility of HPLC results?

A) Column length

B) Detector type

C) Injection volume

D) Column equilibration time

#10. What is the significance of the "peak area" in HPLC analysis?

A) It represents the time taken for a compound to elute.

B) It is used to calculate the concentration of a compound

C) It measures the column temperature.

D) It controls the pump pressure.

#11. What is the purpose of the injection valve in an HPLC system?

A) To control the flow rate of the mobile phase

B) To introduce the sample onto the column

C) To regulate the column temperature

D) To monitor the detector sensitivity

#12. How does the choice of column length and particle size impact the resolution in HPLC analysis?

A) Longer columns improve resolution, while smaller particle sizes decrease it

B) Shorter columns improve resolution, while larger particle sizes decrease it

C) Both longer columns and smaller particle sizes improve resolution

D) Both shorter columns and larger particle sizes improve resolution

#13. What is the role of the stationary phase in reversed-phase HPLC, and how does it interact with analytes?

A) It interacts based on charge, promoting ion exchange

B) It is non-polar and interacts with analytes based on hydrophobicity C) It selectively retains analytes based on their mass

C) It selectively retains analytes based on their mass

D) It facilitates separation by molecular size exclusion

#14. How does changing the pH of the mobile phase impact the separation of acidic and basic compounds in ion-exchange chromatography?

A) It has no effect on separation

B) It enhances separation of acidic compounds

C) It enhances separation of basic compounds

D) It improves separation of both acidic and basic compounds

#15. What is the purpose of the pre-column in an HPLC system, and how does it contribute to method sensitivity?

A) To increase sample throughput

B) To enhance the separation of complex mixtures

C) To remove impurities before reaching the analytical column

D) To control the detector response time

#16. Explain the term "peak asymmetry" in the context of HPLC chromatograms, and what factors can cause asymmetrical peaks?

A) It measures the detector response time

B) It indicates a poorly packed column

C) It describes the tailing or fronting of peaks

D) It is influenced by the injection volume

#17. Why is it important to use a mobile phase filter in an HPLC system, and what issues can arise if the mobile phase is not adequately filtered?

A) To regulate the pump pressure

B) To prevent clogging of the detector

C) To remove particulate matter that may damage the column

D) To control the autosampler speed

#18. How does the dwell volume in the detector cell impact the sensitivity of a UV-Visible detector in HPLC?

A) It increases sensitivity

B) It decreases sensitivity

C) It has no effect on sensitivity

D) It controls the autosampler injection volume

#19. Discuss the advantages of using a multi-wavelength detector in HPLC compared to a single-wavelength detector.

A) Improved sensitivity and reduced baseline noise

B) Increased analysis time and better peak shape

C) Enhanced selectivity and higher column efficiency

D) Lower cost and simplified method development

#20. In what situations would one choose isocratic elution over gradient elution in HPLC, and vice versa?

A) Isocratic for complex mixtures, gradient for simple samples

B) Isocratic for rapid analysis, gradient for improved resolution

C) Isocratic for increased resolution, gradient for quick analysis

D) Isocratic for polar compounds, gradient for non-polar compounds

Finish