#1. What is the primary principle of affinity chromatography?
#2. Which molecule is immobilized on the stationary phase in affinity chromatography?
#3. Which property of a ligand ensures effective separation in affinity chromatography?
#4. What is the typical method used to elute bound molecules in affinity chromatography?
#5. What type of interactions does a ligand form with the target molecule?
#6. Affinity chromatography is often used to purify which biomolecule?
#7. Which of the following ligands is commonly used for purifying immunoglobulins?
#8. Histidine-tagged proteins are purified using columns coated with which ligand?
#9. Which type of affinity chromatography is used for purifying glycoproteins?
#10. What is the role of glutathione in GST-tagged protein purification?
#11. What does the term “specific activity” refer to in affinity chromatography?
#12. To avoid non-specific binding, one should:
#13. Which factor does NOT affect ligand binding in affinity chromatography?
#14. What is the most common elution strategy to recover target proteins?
#15. How can column regeneration be achieved in affinity chromatography?
#16. What is the term for the ability of a ligand to bind only one specific target?
#17. What is the primary advantage of affinity chromatography over other techniques?
#18. Which of these molecules cannot be purified by affinity chromatography?
#19. Immobilized metal ion affinity chromatography (IMAC) is based on the interaction of metals with:
#20. In affinity chromatography, the stationary phase is typically:
#21. What is a major challenge in affinity chromatography?
#22. How can column clogging be minimized?
#23. What is a common detection method for protein purity after chromatography?
#24. How is ligand leakage monitored in the elution fractions?
#25. In large-scale applications, which parameter is most critical for column design?
#26. Affinity chromatography is a subtype of:
#27. What type of binding is used in DNA-affinity chromatography?
#28. What type of molecule is often used for affinity purification of lectins?
#29. What is the typical flow rate in affinity chromatography for efficient binding?
#30. What is the typical flow rate in affinity chromatography for efficient binding?
#31. Which software can model affinity chromatography performance?
