Ion Exchange Chromatography MCQ

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#1. What is the principle of ion exchange chromatography?

A) Ion attraction between stationary and mobile phases

B) Ion repulsion between the stationary phase and the solutes

C) Hydrophobic interactions between ions

D) Separation based on molecular weight

#2. Which of the following is a typical stationary phase in cation exchange chromatography?

A) Positively charged resins

B) Negatively charged resins

C) Neutral beads

D) Hydrophobic membranes

#3. In ion exchange chromatography, anions are exchanged in:

A) Cation exchange chromatography

B) Anion exchange chromatography

C) Both cation and anion exchange chromatography

D) Neither of the above

#4. What type of ions are typically used to elute proteins in ion exchange chromatography?

A) Hydrophobic ions

B) Non-charged molecules

C) High concentration salts

D) Buffers with low ionic strength

#5. What is the main factor influencing the binding strength of molecules in ion exchange chromatography?

A) Size of molecules

B) Electrical charge of molecules

C) pH of the solution only

D) Temperature of the column

#6. What does the term “exchange capacity” refer to in ion exchange chromatography?

A) The volume of the eluent required for separation

B) The number of ions that can bind to the resin

C) The pressure tolerance of the column

D) The molecular weight threshold of molecules

#7. Identify the correct sequence of steps in ion exchange chromatography:

Equilibration

Sample application

Elution

Regeneration A) 4 → 3 → 2 → 1 B) 1 → 2 → 3 → 4 C) 2 → 1 → 4 → 3 D) 3 → 2 → 4 → 1

#8. Which parameter is adjusted to control the separation of compounds in ion exchange chromatography?

A) pH of the buffer

B) Column length

C) Temperature of the system

D) Detector wavelength

#9. Scenario: A protein sample has a pI of 6.5. At pH 8.0, which type of ion exchange resin would it bind to?

A) Cation exchange resin

B) Anion exchange resin

C) Neutral resin

D) No binding would occur

#10. Fill in the blanks: In ion exchange chromatography, the the ionic strength of the eluent, the the interaction between the solute and the resin.

A) Lower; stronger

B) Higher; stronger

C) Higher; weaker

D) Lower; weaker

#11. What is the role of the mobile phase in ion exchange chromatography?

A) Acts as the stationary phase

B) Facilitates ion binding

C) Transports ions and elutes bound molecules

D) None of the above

#12. Ion exchange chromatography is most commonly used for separating which type of molecules?

A) Carbohydrates

B) Lipids

C) Proteins and nucleic acids

D) Steroids

#13. Which method is used to regenerate the ion exchange resin?

A) High heat

B) Acid or base washing

C) Mechanical cleaning

D) UV light exposure

#14. Identify the type of ion exchange chromatography used in desalting proteins:

A) Cation exchange chromatography

B) Anion exchange chromatography

C) Size exclusion chromatography

D) Not applicable

#15. If the pH is equal to the pI of a protein, what is the charge on the protein?

A) Positive

B) Negative

C) Neutral

D) Cannot be determined

#16. If the pH is equal to the pI of a protein, what is the charge on the protein?

A) Positive

B) Negative

C) Neutral

D) Cannot be determined

#17. What property of the stationary phase can affect the resolution in ion exchange chromatography?

A) Pore size

B) Resin density

C) Charge density

D) All of the above

#18. Which elution technique involves a gradual increase in salt concentration?

A) Isocratic elution

B) Gradient elution

C) Pulse elution

D) Step elution

#19. What happens if a buffer with too high a pH is used in cation exchange chromatography?

A) Increased protein binding

B) Decreased protein binding

C) No effect

D) Proteins denature

#20. Which detector is most commonly used with ion exchange chromatography?

A) UV-Vis spectrophotometer

B) Fluorescence detector

C) Conductivity detector

D) Mass spectrometer

#21. Choose the correct order: Resin Functional Groups → Target Molecule → Binding Interaction

A) Negative → Positive → Ionic

B) Positive → Neutral → Ionic

C) Neutral → Negative → Hydrophobic

D) Negative → Negative → Hydrophobic

#22. Ion exchange chromatography can be used to purify DNA due to its:

A) Negative charge

B) Positive charge

C) Neutral charge

D) Hydrophobicity

#23. What is the effect of using a buffer with a high ionic strength in ion exchange chromatography?

A) Stronger binding

B) Faster elution

C) Slower elution

D) Irreversible binding

#24. In ion exchange chromatography, the ______ of the mobile phase determines the strength of the interaction between the analyte and the stationary phase.

A) Temperature

B) Charge

C) Ionic strength

D) pH

#25. What happens to the binding of molecules to a cation exchange resin when the pH of the buffer decreases?

A) Binding increases

B) Binding decreases

C) Binding remains the same

D) Molecules are eluted immediately

Finish

Results

#1. What is the principle of ion exchange chromatography?

#2. Which of the following is a typical stationary phase in cation exchange chromatography?

#3. In ion exchange chromatography, anions are exchanged in:

#4. What type of ions are typically used to elute proteins in ion exchange chromatography?

#5. What is the main factor influencing the binding strength of molecules in ion exchange chromatography?

#6. What does the term “exchange capacity” refer to in ion exchange chromatography?

#7. Identify the correct sequence of steps in ion exchange chromatography:

#8. Which parameter is adjusted to control the separation of compounds in ion exchange chromatography?

#9. Scenario: A protein sample has a pI of 6.5. At pH 8.0, which type of ion exchange resin would it bind to?

#10. Fill in the blanks: In ion exchange chromatography, the the ionic strength of the eluent, the the interaction between the solute and the resin.

#11. What is the role of the mobile phase in ion exchange chromatography?

#12. Ion exchange chromatography is most commonly used for separating which type of molecules?

#13. Which method is used to regenerate the ion exchange resin?

#14. Identify the type of ion exchange chromatography used in desalting proteins:

#15. If the pH is equal to the pI of a protein, what is the charge on the protein?

#16. If the pH is equal to the pI of a protein, what is the charge on the protein?

#17. What property of the stationary phase can affect the resolution in ion exchange chromatography?

#18. Which elution technique involves a gradual increase in salt concentration?

#19. What happens if a buffer with too high a pH is used in cation exchange chromatography?

#20. Which detector is most commonly used with ion exchange chromatography?

#21. Choose the correct order: Resin Functional Groups → Target Molecule → Binding Interaction

#22. Ion exchange chromatography can be used to purify DNA due to its:

#23. What is the effect of using a buffer with a high ionic strength in ion exchange chromatography?

#24. In ion exchange chromatography, the ______ of the mobile phase determines the strength of the interaction between the analyte and the stationary phase.

#25. What happens to the binding of molecules to a cation exchange resin when the pH of the buffer decreases?

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Results

#1. What is the principle of ion exchange chromatography?

#2. Which of the following is a typical stationary phase in cation exchange chromatography?

#3. In ion exchange chromatography, anions are exchanged in:

#4. What type of ions are typically used to elute proteins in ion exchange chromatography?

#5. What is the main factor influencing the binding strength of molecules in ion exchange chromatography?

#6. What does the term “exchange capacity” refer to in ion exchange chromatography?

#7. Identify the correct sequence of steps in ion exchange chromatography:

#8. Which parameter is adjusted to control the separation of compounds in ion exchange chromatography?

#9. Scenario: A protein sample has a pI of 6.5. At pH 8.0, which type of ion exchange resin would it bind to?

#10. Fill in the blanks: In ion exchange chromatography, the ______ the ionic strength of the eluent, the ______ the interaction between the solute and the resin.

#11. What is the role of the mobile phase in ion exchange chromatography?

#12. Ion exchange chromatography is most commonly used for separating which type of molecules?

#13. Which method is used to regenerate the ion exchange resin?

#14. Identify the type of ion exchange chromatography used in desalting proteins:

#15. If the pH is equal to the pI of a protein, what is the charge on the protein?

#16. If the pH is equal to the pI of a protein, what is the charge on the protein?

#17. What property of the stationary phase can affect the resolution in ion exchange chromatography?

#18. Which elution technique involves a gradual increase in salt concentration?

#19. What happens if a buffer with too high a pH is used in cation exchange chromatography?

#20. Which detector is most commonly used with ion exchange chromatography?

#21. Choose the correct order: Resin Functional Groups → Target Molecule → Binding Interaction

#22. Ion exchange chromatography can be used to purify DNA due to its:

#23. What is the effect of using a buffer with a high ionic strength in ion exchange chromatography?

#24. In ion exchange chromatography, the ______ of the mobile phase determines the strength of the interaction between the analyte and the stationary phase.

#25. What happens to the binding of molecules to a cation exchange resin when the pH of the buffer decreases?

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#10. Fill in the blanks: In ion exchange chromatography, the the ionic strength of the eluent, the the interaction between the solute and the resin.