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MCQ of Size exclusion chromatography

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Results

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#1. What is the primary principle behind size exclusion chromatography?

A) Charge separation

B) Size separation

C) Affinity interactions

D) Hydrophobic interactions

#2. Which of the following molecules elutes first in size exclusion chromatography?

A) Small molecules

B) Large molecules

C) Medium molecules

D) Molecules with high charge

#3. What is the role of the stationary phase in SEC?

A) Attract molecules based on charge

B) Exclude molecules based on size

C) Bind specific molecules

D) Separate molecules based on hydrophobicity

#4. The term “void volume” in SEC refers to:

) The volume of solvent inside the pores

B) The volume of solvent outside the pores

C) The total column volume

D) The volume of solute injected

#5. Which polymer is commonly used as the stationary phase in SEC?

A) Agarose

B) Silica

C) Cellulose

D) Dextran

#6. Size exclusion chromatography is best suited for separating:

A) Proteins and peptides

B) Nucleotides

C) Lipids

D) Small organic molecules

#7. What is the main mobile phase used in SEC for biomolecules?

A) Water

B) Organic solvents

C) Buffer solutions

D) None of the above

#8. Which of the following is a critical factor for SEC efficiency?

A) Flow rate

B) pH of the mobile phase

C) Ionic strength

D) All of the above

#9. How does increasing the flow rate affect SEC resolution?

A) Increases resolution

B) Decreases resolution

C) No effect

D) Improves peak sharpness

#10. What type of detector is commonly used in SEC?

A) UV-Vis

B) Refractive index detector

C) Light scattering detector

D) All of the above

#11. In SEC, what is the retention factor (k)?

A) Ratio of void volume to total column volume

B) Time a solute spends in the stationary phase

C) Ratio of elution volume to total column volume

D) Fraction of solute in the mobile phase

#12. Which factor primarily determines resolution in SEC?

A) Column length

B) Pore size distribution

C) Sample concentration

D) All of the above

#13. What is another name for Size Exclusion Chromatography?

A) Ion-exchange chromatography

B) Gel filtration chromatography

C) Affinity chromatography

D) Paper chromatography

#14. Which of the following best describes the stationary phase material used in SEC?

A) Porous beads

B) Glass fibers

C) Silica plates

D) Non-porous resins

#15. In SEC, which factor does NOT significantly influence separation?

A) Molecular weight

B) Molecular shape

C) Solvent polarity

D) Pore size of the stationary phase

#16. What is the term for the range of molecular sizes that can be separated in SEC?

A) Separation zone

B) Fractionation range

C) Exclusion volume

D) Pore limit

#17. If a molecule is larger than the largest pore size, it will:

A) Elute at the void volume

B) Penetrate partially into the pores

C) Be retained longer in the column

D) Elute last

#18. What shape is assumed for proteins during molecular weight determination via SEC?

A) Linear chain

B) Globular

C) Helical

D) Random coil

#19. Which component is essential for accurate size exclusion chromatography?

A) High-pressure pumps

B) Gradient elution system

C) Column with uniform pore size

D) Strongly binding stationary phase

#20. Which detector provides molecular weight information directly in SEC?

A) UV-Vis spectrophotometer

B) Light scattering detector

C) Fluorescence detector

D) Electrochemical detector

#21. What type of flow rate is generally optimal for SEC?

A) High flow rate for quick separations

B) Low and steady flow rate for better resolution

C) Pulsating flow rate for enhanced mixing

D) None of the above

#22. Which macromolecule is most commonly analyzed by SEC?

A) Lipids

B) Proteins

C) Amino acids

D) Sugars

#23. Which of the following methods can be coupled with SEC for better analysis?

A) Mass spectrometry

B) Nuclear magnetic resonance

C) Circular dichroism

D) Infrared spectroscopy

Results

#1. What is the primary principle behind size exclusion chromatography?

#2. Which of the following molecules elutes first in size exclusion chromatography?

#3. What is the role of the stationary phase in SEC?

#4. The term “void volume” in SEC refers to:

#5. Which polymer is commonly used as the stationary phase in SEC?

#6. Size exclusion chromatography is best suited for separating:

#7. What is the main mobile phase used in SEC for biomolecules?

#8. Which of the following is a critical factor for SEC efficiency?

#9. How does increasing the flow rate affect SEC resolution?

#10. What type of detector is commonly used in SEC?

#11. In SEC, what is the retention factor (k)?

#12. Which factor primarily determines resolution in SEC?

#13. What is another name for Size Exclusion Chromatography?

#14. Which of the following best describes the stationary phase material used in SEC?

#15. In SEC, which factor does NOT significantly influence separation?

#16. What is the term for the range of molecular sizes that can be separated in SEC?

#17. If a molecule is larger than the largest pore size, it will:

#18. What shape is assumed for proteins during molecular weight determination via SEC?

#19. Which component is essential for accurate size exclusion chromatography?

#20. Which detector provides molecular weight information directly in SEC?

#21. What type of flow rate is generally optimal for SEC?

#22. Which macromolecule is most commonly analyzed by SEC?

#23. Which of the following methods can be coupled with SEC for better analysis?

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