Thin Layer Chromatography TLC MCQ » Flair Pharma The Knowledge Kit.

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#1. 1. What does TLC stand for in analytical chemistry?

A) Total Liquid Chromatography

B) Thin Layer Chromatography

C) Temperature Layer Chromatography

D) Transient Layer Chromatography

#2. What is the primary purpose of TLC?

A) To separate gas mixtures

B) To separate liquid mixtures

C) To separate solid mixtures

D) To separate and identify components of a mixture

#3. Which of the following is used as a stationary phase in TLC?

A) Gas

B) Liquid

C) Solid

D) Gel

#4. In TLC, what is the mobile phase typically composed of?

A) A solid

B) A gas

C) A liquid solvent or solvent mixture

D) A gel

#5. What is the main advantage of TLC?

A) High cost

B) Complexity

C) Speed and simplicity

D) Requires large sample sizes

#6. Which type of substances can be analyzed using TLC?

A) Only polar compounds

B) Only nonpolar compounds

C) Both polar and nonpolar compounds

D) Only gases

#7. What does Rf value stand for in TLC?

A) Relative factor

B) Retention factor

C) Reference factor

D) Refractive factor

#8. How is the Rf value calculated?

A) Distance traveled by the solvent / distance traveled by the solute

B) Distance traveled by the solute / distance traveled by the solvent

C) Distance traveled by the stationary phase / distance traveled by the mobile phase

D) Distance traveled by the solvent - distance traveled by the solute

#9. What happens if the solvent front is not allowed to reach the top of the TLC plate?

A) The results are more accurate

B) The separation is incomplete

C) The Rf values are higher

D) The compounds cannot be visualized

#10. Which of the following is a common visualization technique for TLC plates?

A) Mass spectrometry

B) Ultraviolet light

C) Infrared spectroscopy

D) Gas chromatography

#11. What role does the solvent play in TLC?

A) It binds with the stationary phase

B) It acts as the stationary phase

C) It carries the sample components up the plate

D) It prevents sample diffusion

#12. In TLC, what is the purpose of using a developing chamber?

A) To heat the plate

B) To provide a controlled environment for solvent evaporation

C) To keep the plate horizontal

D) To ensure proper solvent saturation

#13. Which factor does NOT affect Rf values in TLC?

A) Solvent polarity

B) Temperature

C) Thickness of the stationary phase

D) Color of the solvent

#14. What is typically used as the stationary phase in a TLC plate?

A) Silica gel

B) Sodium chloride

C) Water

D) Ethanol

#15. Which of the following can be used as a mobile phase in TLC?

A) Methanol

B) Water

C) HexaneX

D) All of the above

#16. In a TLC experiment, how is the sample usually applied to the plate?

A) By soaking the plate in the sample

B) By spotting the sample onto the plate

C) By spraying the plate with the sample

D) By immersing the plate in the sample

#17. Why is it important to avoid touching the surface of a TLC plate?

A) It can cause contamination

B) It can lead to evaporation of solvents

C) It makes the plate fragile

D) It increases the cost of the experiment

#18. What is the typical thickness of a TLC plate?

A) 0.1 mm

B) 0.5 mm

C) 1 mm

D) 0.2 mm

#19. Which of the following statements about TLC is FALSE?

A) TLC can be used for both qualitative and quantitative analysis.

B) TLC requires large sample sizes for analysis.

C) Rf values are characteristic for each compound under specific conditions.

D) The solvent front should be marked immediately after developing the plate.

#20. Which parameter is crucial for selecting the mobile phase in TLC?

A) Sample color

B) Sample temperature

C) Polarity of the compounds to be separated

D) Molecular weight of the compounds

#21. What is a common use of TLC in the pharmaceutical industry?

A) To determine the molecular weight of compounds

B) To identify impurities in a compound

C) To measure the melting point of substances

D) To quantify the exact concentration of a drug

#22. In TLC, what does it mean if multiple spots appear for a single sample?

A) The sample is pure

B) The sample has multiple components

C) The solvent was too polar

D) The TLC plate is faulty

#23. Which type of TLC is specifically designed for analyzing volatile compounds?

A) High-Performance TLC

B) Normal Phase TLC

C) Reverse Phase TLC

D) Gas-Liquid TLC

#24. What is the role of a reference standard in TLC?

A) To increase the Rf value

B) To act as a solvent

C) To help identify and compare the components of a sample

D) To improve the visualization of spots

#25. Which of the following is an advantage of using TLC over other chromatographic techniques?

A) Requires sophisticated equipment

B) Can handle only small samples

C) Faster results with minimal preparation

D) More expensive than HPLC

#26. What happens if the solvent front is allowed to rise too high on the TLC plate?

A) The compounds will not separate effectively

B) The compounds may run off the plate

C) The Rf values will decrease

D) The plate will become damaged

#27. What type of chromatography is TLC often compared to?

A) High-Performance Liquid Chromatography (HPLC)

B) Gas Chromatography (GC)

C) Ion Chromatography

D) Affinity Chromatography

#28. What is one limitation of TLC?

A) It is time-consuming

B) It provides qualitative but not quantitative data

C) It cannot analyze polar compounds

D) It requires large amounts of solvent

#29. Which is a key component of the TLC process?

A) A solid stationary phase and a liquid mobile phase

B) A liquid stationary phase and a gas mobile phase

C) A gas stationary phase and a liquid mobile phase

D) A gel stationary phase and a solid mobile phase

#30. What is the main purpose of using a UV lamp after developing a TLC plate?

A) To dry the plate

B) To visualize and identify spots of certain compounds

C) To change the color of the compounds

D) To determine the weight of the compounds

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